Intended Use:
Recommended for the cultivation of fastidious pathogenic bacteria, yeasts and moulds from clinical and non clinical samples.
Composition
(BS) Ingredients Gms / Litre
HM infusion powder # 12.500
BHI powder 5.000
Proteose peptone 10.000
Dextrose (Glucose) 2.000
Sodium chloride 5.000
Disodium hydrogen phosphate 2.500
Agar 15.000
Final pH ( at 25°C) 7.4±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Calf brain infusion from
Directions
Suspend 52.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates. If desired, 20 units Penicillin and 40 µg Streptomycin per ml of medium may be added to make the medium selective for fungi.
Storage and Shelf Life
Store between 10- 30°C in a tightly closed container and the prepared medium at 2 – 8°C. Use before expiry date on the label.
