Blood Agar Base No. 2 , 500g , Himedia™
Blood Agar Base No. 2 medium after addition of blood, medium permits maximum recovery of Streptococci, Pneumococci and other fastidious pathogenic microorganisms without interfering with their haemolytic reactions. It is a highly nutritive medium. Microorganisms producing haemolysin give visible haemolytic zones on this medium. It also serves as a differential medium for Brucella and Campylobacter species by adding different antibiotics supplements for the respective bacteria. Also it can be used to prepare Chocolate Agar for the isolation of Haemophilus and Neisseria species, where horse blood is used for enrichment. Better results are obtained by spreading half of the horse blood agar plate with 2 drops of saponin.
Blood Agar Base No. 2 (M834)
Intended Use:
After addition of blood, medium permits maximum recovery of Streptococci, Pneumococci and other fastidious pathogenic microorganisms without interfering with their haemolytic reactions.
Composition
(BS) Ingredients Gms / Litre
Proteose peptone 15.000
HL extract # 2.500
Yeast extract 5.000
Sodium chloride 5.000
Agar 15.000
Final pH ( at 25°C) 7.4±0.2
**Formula adjusted, standardized to suit performance parameters
# -Equivalent to Liver extract
Directions
Suspend 21.25 grams in 500 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45 – 50°C and aseptically add 7% v/v sterile defibrinated blood.
For Brucella species: Add rehydrated contents of 1 vial of NPBCVN Selective Supplement (FD005) to 500 ml sterile molten base.
For Campylobacter species : Add rehydrated contents of 1 vial of Blaser-Wang Selective Supplement (FD006) or Butzler Selective Supplement (FD007) or Skirrow Selective Supplement (FD008) or Minerals Growth Supplement (FD009) to 500 ml sterile molten base.
For Streptococcus species: Add rehydrated contents of 1 vial of NNP Selective Supplement (FD031) to 500 ml sterile molten base. Mix well and pour into sterile Petri plates.
Storage and Shelf Life
Store between 10- 30°C in a tightly closed container and the prepared medium at 2 – 8°C. Use before expiry date on the label.
