Intended Use:
Recommended for isolation and cultivation of all Lactobacilli from food, dairy and clinical samples.
Composition
Ingredients g/ L
Proteose peptone 10.000
HM Peptone B # 10.000
Yeast extract 5.000
Dextrose (Glucose) 20.000
Tween 80 (Polysorbate 80) 1.000
Ammonium citrate 2.000
Sodium acetate 5.000
Magnesium sulphate 0.100
Manganese sulphate 0.050
Dipotassium hydrogen phosphate 2.000
Agar 12.000
Final pH ( at 25°C) 6.5±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Suspend 67.15 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by
autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Storage and Shelf Life
Store dehydrated powder and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product
should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic
nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected
from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used
within stated expiry period.
