Brilliant Green Bile Broth, 2%, 500g , Himedia™
Recommended for detection and confirmation of coliform bacteria in water, waste water, food, milk and dairy products.
Brilliant Green Bile Broth 2% (M121)
Intended Use:
Recommended for detection and confirmation of coliform bacteria in water, waste water, food, milk and dairy products.
Composition
Ingredients g / L
Peptone 10.000
Lactose 10.000
Bile# 20.000
Brilliant green 0.0133
Final pH ( at 25°C) 7.2±0.2
**Formula adjusted, standardized to suit performance parameters
# – Equivalent to Oxgall
Directions
Suspend 40.01 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Distribute in
fermentation tubes containing inverted Durhams tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
For preparation of double strength it is recommended to heat the dissolved broth (80.02 grams per litre) at 100°C for 30
minutes. Do not autoclave double strength broth.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label.
