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Food Pathogen

Choose your Reference Method

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Conventional Method

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Collect food sample & swab aseptically

Homogenize sample

(e.g., with stomacher)

Dilute in sterile buffer (1:10)

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Add food sample to non-selective pre-enrichment broth

Incubate at target temperature

(e.g., 35–37°C) for 16–24 hours

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Transfer aliquot to selective enrichment broth

Further incubation

(e.g., 24–48 hours)

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Streak on selective agar plates

Incubate at appropriate temperature and time

Observe colony morphology

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Biochemical tests

Immunological assays

(e.g., with stomacher)

Molecular methods (Real Time PCR)

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