EMB Agar, Levine (M022)
Intended Use:
Recommended for the isolation, enumeration and differentiation of members of Enterobacteriaceae from clinical and non clinical samples.
Composition
Ingredients g/ L
Peptone 10.000
Dipotassium hydrogen phosphate 2.000
Lactose 10.000
Eosin – Y 0.400
Methylene blue 0.065
Agar 15.000
Final pH ( at 25°C) 7.1±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 37.46 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by
autoclaving at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING. Cool to 50°C and shake the medium in
order to oxidize the methylene blue (i.e. restore its blue colour) and to suspend the precipitate, which is an essential part of
the medium.
Precaution : Store the medium away from light to avoid photo-oxidation.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on
the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump
formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store
in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use.
Product performance is best if used within stated expiry period.
