Deoxycholate Agar (M030)
Intended Use:
Deoxycholate Agar is used as a differential medium for the direct count of coliforms in dairy products. Also used for the isolation of enteric pathogens from rectal swabs, faeces and other pathological specimens.
Composition
Ingredients g/ L
Peptone 10.000
Lactose 10.000
Sodium deoxycholate 1.000
Sodium chloride 5.000
Dipotassium hydrogen phosphate 2.000
Ferric citrate 1.000
Sodium citrate 1.000
Neutral red 0.030
Agar 15.000
Final pH ( at 25°C) 7.3±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 45.03 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. DO NOT
AUTOCLAVE. Avoid excessive or prolonged heating during reconstitution. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the
label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation
due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry
ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product
performance is best if used within stated expiry period.
