Nutrient Broth , 500G , Himedia™

Nutrient Broth                                           ( M002 )

Intended use 
Nutrient Broth is used for the general cultivation of less fastidious microorganisms, can be enriched with blood or other biological fluids.

Composition**
Ingredients                                                                  g / L
Peptone                                                                       5.000
Sodium chloride                                                          5.000
HM peptone B#                                                           1.500
Yeast extract                                                               1.500
Final pH ( at 25°C)                                                     7.4±0.2
**Formula adjusted, standardized to suit performance parameters
# – Equivalent to Beef extract

Directions 
Suspend 13.0 grams in 1000 ml purified / distilled water. Heat, if necessary, to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes

Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent solution
Reaction
Reaction of 1.3% w/v aqueous solution at 25°C. pH : 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label.