MR-VP Medium , 500G , Himedia™

MR-VP Medium (Buffered Glucose Broth)(Glucose Phosphate Broth),Granulated               ( GM070 )

Intended use 
Recommended for performance of Methyl Red and Voges-Proskauer tests in differentiation of coli-aerogenes group.

Composition**
Ingredients                                                                     g / L
Buffered peptone                                                            7.000
Dextrose (Glucose)                                                        5.000
Dipotassium hydrogen phosphate                                 5.000
Final pH ( at 25°C)                                                       6.9±0.2
**Formula adjusted, standardized to suit performance parameters

Directions 
Suspend 17.0 grams in 1000 ml of purified/distilled water. Heat if necessary to dissolve the medium completely. Distribute in test tubes in 10 ml amounts or as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Quality Control
Appearance
Cream to yellow coloured granular medium
Colour and Clarity of prepared medium
Light yellow coloured clear solution without any precipitate
Reaction
Reaction of 1.7% w/v aqueous solution at 25°C. pH : 6.9±0.2
pH
6.70-7.10
Cultural Response
Cultural characteristics observed after an incubation at 30-32°C for 18-48 hours.

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2 – 8°C. Use before expiry date on the label.