Motility Nitrate Medium, Buffered , 500G , Himedia™

Motility Nitrate Medium, Buffered                              ( M630 )

Intended use 
Recommended for isolation and detection of Clostridium perfringens on the basis of motility and nitrate test.

*Composition**
Ingredients                                                             Gms / Litre
Peptone                                                                       5.000
HM peptone B #                                                          3.000
Galactose                                                                    5.000
Potassium nitrate                                                        1.000
Disodium hydrogen phosphate                                   2.500
Agar                                                                            3.000
Final pH ( at 25°C)                                                     7.4±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract

Directions 
Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium completely. Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool quickly in cool running water and allow the tubed medium to solidify in an upright position.

Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Semisolid, comparable with 0.3% Agar gel.
Colour and Clarity of prepared medium
Light amber coloured clear to slightly opalescent gel forms in tubes as butts
Reaction
Reaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours .

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label.