Lysine Decarboxylase Broth , 500g , Himedia™

Lysine Decarboxylase Broth                      ( M376 )

Intended use 
Recommended for differentiating Salmonella arizonae from the Bethesda Ballerup group of Enterobacteriaceae.

Composition**
Ingredients                                                  Gms / Litre
Peptone                                                              5.000
Yeast extract                                                       3.000
Dextrose (Glucose)                                            1.000
L-Lysine hydrochloride                                       5.000
Bromocresol purple                                            0.020
Final pH ( at 25°C)                                            6.8±0.2

**Formula adjusted, standardized to suit performance parameters

Directions
Suspend 14.02 grams in 1000 ml purified / distilled water. Heat, if necessary to dissolve the medium completely. Dispense 5 ml amount into screw-capped test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubed medium in an upright position and overlay with 2-3 ml of sterile mineral oil..

Quality Control
Appearance
Light yellow to greenish yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Purple coloured clear solution without any precipitate
Reaction
Reaction of 1.4% w/v aqueous solution at 25°C. pH : 6.8±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. (Inoculated tubes are overlayed with sterile mineral oil).

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date
on the label.