Lactobacillus MRS Broth (MRS Broth) , 500g , Himedia™

Lactobacillus MRS Broth  ( GM369 )

Intended use
Recommended for cultivation of all Lactobacilli from clinical and non clinical samples.

Composition**
Ingredients                                       Gms / Litre
Proteose peptone                                10.000
HM Peptone B#                                   10.000
Yeast extract                                         5.000
Dextrose(Glucose)                              20.000
Polysorbate 80 (Tween 80)                  1.000
Ammonium citrate                                2.000
Sodium acetate                                    5.000
Magnesium sulphate                            0.100
Manganese sulphate                            0.050
Dipotassium hydrogen phosphate       2.000
Final pH ( at 25°C)                             6.5±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef Extract

Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light amber coloured, clear to slightly opalescent solution in tubes
Reaction
Reaction of 5.51% w/v aqueous solution at 25°C. pH : 6.5±0.2
pH
6.30-6.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours or longer (with 5% CO2)

Storage and Shelf Life
Store dehydrated and the prepared medium at 2-8°C. Use before expiry date on the label.