The Bionova® BT30 Biological Indicator provides a 48-hour readout for dry heat sterilization monitoring at 160–180 °C. Contains Bacillus atrophaeus spores (10⁶) for accurate, reliable sterility assurance in labs, hospitals, and industrial settings.
The Bionova® BT30 Semi Self-Contained Biological Indicator (SCBI) is designed for accurate monitoring of dry heat sterilization processes at 160–180 °C. Each indicator contains Bacillus atrophaeus (ATCC® 9372) with 10⁶ spores per vial, providing a reliable challenge to sterilization cycles.
Key Features & Benefits
✅ 48-hour readout for precise sterility confirmation
🔬 High spore population ensures accurate validation of dry heat sterilization
🎯 Designed for critical areas of the load (center or near the door)
⚡ Compatible with standard incubators at 37 ± 2 °C
✔ Positive control included to verify incubation conditions
How It Works
Place the BT30 indicator in the most challenging areas of the sterilizer load.
Run the dry heat sterilization cycle at 160–180 °C as usual.
After sterilization, carefully break the ampoule in tube B to allow contact between the spore carrier and culture medium in tube A.
Incubate at 37 ± 2 °C for a maximum of 48 hours.
Observe color change:
Blue → Yellow indicates sterilization failure (viable spores present)
Remains Blue confirms successful sterilization
Positive control must turn yellow to validate results.
Directions & Safety Notes
Follow laminar flow or sterile environment procedures when handling ampoules and spore carriers.
Avoid exposure to temperatures above 180 °C or mechanical damage that could release spores externally.
Use the BT30 in combination with dual incubators (e.g., Bionova® IC10/20) for accurate temperature control.
Technical Data
Readout: 48 hours
Incubation: 37 ± 2 °C
Organism: Bacillus atrophaeus ATCC® 9372
Spores per vial: 10⁶
Sterilization range: 160–180 °C
Compliance: Meets international dry heat sterilization monitoring standards
The Bionova® BT30 ensures reliable monitoring of dry heat sterilization, providing laboratories, hospitals, and industrial facilities with confidence in infection control and process validation.
