{"id":8483,"date":"2026-04-21T10:38:39","date_gmt":"2026-04-21T03:38:39","guid":{"rendered":"https:\/\/bcst.co.th\/product\/terragene-biological-indicator-dry-heat-sterilization-thailand\/"},"modified":"2026-04-21T10:38:39","modified_gmt":"2026-04-21T03:38:39","slug":"terragene-biological-indicator-dry-heat-sterilization-thailand","status":"publish","type":"product","link":"https:\/\/bcst.co.th\/th\/product\/terragene-biological-indicator-dry-heat-sterilization-thailand\/","title":{"rendered":"Terragene Bionova BT30 \u2014 Biological Indicator for Dry Heat Sterilization"},"content":{"rendered":"

The Terragene Bionova BT30 biological indicator for dry heat sterilization reaches Thailand through BCST, providing laboratories, glassware sterilization operations, pharmaceutical manufacturers, and dental facilities using hot-air ovens with a validated biological indicator specifically engineered for dry heat sterilization processes.<\/em><\/p>\n

Biological Indicator Dry Heat Thailand \u2014 Why Dry Heat Requires A Different Indicator<\/h2>\n

Steam and dry heat are fundamentally different sterilization mechanisms. Steam kills microorganisms through moist heat \u2014 hydrolysis of proteins at relatively lower temperatures. Dry heat kills through oxidation \u2014 a different physical process requiring significantly higher temperatures and longer exposure times to achieve the same level of sterility assurance.<\/p>\n

Consequently, a steam biological indicator cannot be used for dry heat monitoring. Geobacillus stearothermophilus<\/em> \u2014 the challenge organism in steam BIs \u2014 is not the appropriate organism for dry heat validation. Furthermore, the physical format of a steam SCBI cannot withstand the 160\u2013180\u00b0C temperatures required for dry heat sterilization.<\/p>\n

The BT30 uses Bacillus atrophaeus<\/em> ATCC\u00ae 9372 \u2014 the internationally recognized challenge organism for dry heat sterilization monitoring. This organism is more resistant to dry heat than G. stearothermophilus<\/em> is to steam. Additionally, the two-tube system separates the spore-carrying component from the culture medium, allowing only the spore carrier to enter the hot-air oven while the medium remains protected.<\/p>\n

The Two-Tube System Explained<\/h4>\n

The BT30 consists of two physically separate components.<\/p>\n

Tube A contains<\/em> B. atrophaeus spores inoculated on a paper strip attached to the cap. It enters the hot-air oven together with the load. Tube B contains a glass ampoule of blue growth indicator medium. Unlike Tube A, it never enters the oven.<\/em>. It remains outside and protected throughout the sterilization cycle. Furthermore, Tube B must never be exposed to dry heat \u2014 doing so would destroy the culture medium required for the biological readout.<\/p>\n

After sterilization and cooling, the two tubes combine under aseptic conditions. The spore carrier paper from Tube A contacts the medium in Tube B during incubation. If surviving spores are present, they produce metabolic activity that changes the medium from blue to yellow \u2014 a clear, unambiguous positive result indicating sterilization failure. If sterilization was successful, the medium remains blue after 48 hours.<\/p>\n

Directions For Use<\/h4>\n

Step 1<\/strong> \u2014 Label Tube A with the sterilizer number, load number, and processing date.<\/p>\n

Step 2<\/strong> \u2014 Pack Tube A with the materials to be sterilized in an appropriate package. Place the package in the most inaccessible areas of the hot-air oven \u2014 typically the center of the load and near the door. Do not place Tube B in the oven.<\/p>\n

Step 3<\/strong> \u2014 Sterilize at 160\u2013180\u00b0C as usual.<\/p>\n

Step 4<\/strong> \u2014 After the cycle completes, open the oven door and allow materials and Tube A to cool completely to room temperature. The chemical indicator on the label changes to brown, confirming heat exposure.<\/p>\n

Step 5<\/strong> \u2014 Under aseptic conditions (laminar flow or near a flame), break the ampoule inside Tube B by pressing on the bottom of the tube. Remove the cap from Tube B carefully, taking the tube by its base.<\/p>\n

Step 6<\/strong> \u2014 Remove the cap from Tube A, taking the tube by its base. Insert the cap of Tube A into Tube B so that the spore carrier paper contacts the culture medium. Ensure the carrier paper does not touch the exterior of Tube B or any other surface. Cap tightly.<\/p>\n

Step 7<\/strong> \u2014 Incubate at 37 \u00b1 2\u00b0C for a maximum of 48 hours. Read results at convenient 10-hour intervals.<\/p>\n

Step 8<\/strong> \u2014 Always incubate a non-sterilized BT30 as a positive control simultaneously. The positive control must produce a yellow color change \u2014 confirming that the medium supports growth and incubation conditions are adequate.<\/p>\n

Step 9<\/strong> \u2014 A blue-to-yellow color change in the processed indicator at any reading point is a sterilization failure. Do not use the sterilizer until the cause is identified and resolved. A medium that remains blue at 48 hours confirms sterilization success.<\/p>\n

Important:<\/strong> If the product is exposed to temperatures above 180\u00b0C or subjected to mechanical impact, the spore carrier may detach from the cap. In this case, transfer the carrier to the culture medium using a sterile clamp in a sterile environment under laminar flow or close to a flame.<\/p>\n

Product Specifications<\/h4>\n\n
\n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n
\n Specification <\/td>\n \n Detail <\/td>\n <\/tr>\n
\n SKU <\/td>\n \n TER-BT30 <\/td>\n <\/tr>\n
\n Spore organism <\/td>\n \n Bacillus atrophaeus ATCC\u00ae 9372 <\/td>\n <\/tr>\n
\n Sterilisation method <\/td>\n \n Dry heat, 160\u2013180\u00b0C <\/td>\n <\/tr>\n
\n Format <\/td>\n \n Two-tube system (Tube A + Tube B) <\/td>\n <\/tr>\n
\n Readout method <\/td>\n \n Visual colour change \u2014 blue to yellow <\/td>\n <\/tr>\n
\n Readout time <\/td>\n \n 48 hours maximum <\/td>\n <\/tr>\n
\n Incubation temperature <\/td>\n \n 37 \u00b1 2\u00b0C <\/td>\n <\/tr>\n
\n Equipment required <\/td>\n \n Standard incubator at 37\u00b0C <\/td>\n <\/tr>\n
\n Auto-reader required <\/td>\n \n No <\/td>\n <\/tr>\n
\n Process indicator <\/td>\n \n Yes \u2014 changes to brown on heat exposure <\/td>\n <\/tr>\n
\n Positive result (failure) <\/td>\n \n Blue medium changes to yellow <\/td>\n <\/tr>\n
\n Negative result (success) <\/td>\n \n Medium remains blue at 48 hours <\/td>\n <\/tr>\n
\n Storage <\/td>\n \n 10\u201330\u00b0C, 30\u201380% relative humidity, dark place <\/td>\n <\/tr>\n
\n Shelf life <\/td>\n \n 2 years <\/td>\n <\/tr>\n
\n Brand <\/td>\n \n Terragene <\/td>\n <\/tr>\n
\n Supplier <\/td>\n \n BCST \u2014 Biochem Scitech Co., Ltd. <\/td>\n <\/tr>\n <\/table>\n<\/div>