Mueller Hinton Broth , 500G , Himedia™

Mueller Hinton Broth                                  ( M391 )

Intended use 
Recommended for determination of Invitro susceptibility of bacterial strains against antibacterial agents by broth dilution methods.

Composition**
Ingredients                                                                  g / L
HM infusion B from 300 g #                                         2.000
Acicase™$                                                                 17.500
Starch                                                                          1.500
Final pH ( at 25°C)                                                     7.3±0.1
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef, infusion from
$ Equivalent to Casein acid hydrolysate

Directions 
Suspend 21.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Mix well and dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Note : It is suggested to boil the medium before autoclaving to avoid settling of starch at the bottom.

Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light amber coloured clear solution in tubes
Reaction
Reaction of 2.1% w/v aqueous solution at 25°C. pH : 7.3±0.1
pH
7.20-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label.