Mueller Hinton Agar , 500G , Himedia™

Mueller Hinton Agar                                  ( M173 )

Intended use 
Recommended for determination of susceptibility of microorganisms to antimicrobial agents isolated from clinical samples.

Composition**
Ingredients                                                                  g / L
HM infusion solids B # (from 300g)                              2.000
Acicase ##                                                                  17.500
Starch                                                                           1.500
Agar                                                                            17.000
Final pH ( at 25°C)                                                     7.3±0.1
**Formula adjusted, standardized to suit performance parameters
# – Equivalent to Beef heart infusion
## – Equivalent to Casein acid hydrolysate

Directions 
Suspend 38.0 grams in 1000 ml purified/ distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates. Note: The performance of this batch has been tested and standardised as per the current CLSI (formerly, NCCLS) document M6-protocols for Evaluating Dehydrated Mueller Hinton Agar.

Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.7% agar gel.
Colour and Clarity of prepared medium
Light amber coloured clear to slightly opalescent gel froms in Petri plates
Reaction
Reaction of 3.8% w/v aqueous solution at 25°C. pH : 7.3±0.1
pH
7.20-7.40
Cultural Response
Antibiotic susceptibility tests are performed in accordance with, and meet the acceptance limits of the current ISO/TS16782 (15). Performance of the medium is checked in accordance with the CLSI/ EUCAST guidelines.

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label.