Lauryl Sulphate Broth ( Lauryl Tryptose Broth, Lauryl Sulfate Broth ) (M080)
Intended Use:
Recommended for detection and enumeration of coliform bacteria in water, waste water, dairy products ,other food
samples. The composition and performance criteria are as per the specification laid down in ISO 11133:2014 & Amd :2018
Composition
Ingredients g/ L
Tryptose 20.000
Lactose 5.000
Sodium chloride 5.000
Dipotassium hydrogen phosphate 2.750
Potassium dihydrogen phosphate 2.750
Sodium lauryl sulphate (SLS) 0.100
Final pH ( at 25°C) 6.8±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 35.60 gram in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Distribute
into tubes containing inverted Durhams tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For
inoculum of 1 ml or less, use single strength medium. For inocula of 10 ml or more, double strength or proportionate medium
should be prepared.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on
the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump
formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation.
Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly
after use. Product performance is best if used within stated expiry period.
