Lactose Gelatin Medium (M628)
Intended Use:
Recommended for detection of Clostridium species from food samples.
Composition
Ingredients g/ L
Lactose 10.000
Disodium hydrogen phosphate 5.000
Gelatin 120.000
Phenol red 0.050
Final pH ( at 25°C) 7.5±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 135.0 grams in 1000 ml warm purified / distilled water. Heat if necessary to dissolve the medium completely
and dispense 10 ml amounts in 15×150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C)
for 15 minutes. Just before use, heat to boiling to remove dissolved oxygen and cool rapidly to incubation temperature.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the
label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation
due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry
ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product
performance is best if used within stated expiry period.
