Lactose Broth (M1003)
Intended Use:
Recommended for the detection of Enterobacteriaceae particularly coliform and Salmonella species from water, food and dairy products as per Standard Methods and FDA BAM.
Composition
Ingredients g/ L
Peptone 5.000
HM Peptone B# 3.000
Lactose 5.000
Final pH ( at 25°C) 6.9±0.2
**Formula adjusted, standardized to suit performance parameters
# – Equivalent to Beef extract
Directions
Suspend 13.0 grams in 1000 ml purified/ distilled water. Heat if necessary to dissolve the medium completely. For larger
inocula (10 ml or more), concentrated medium may be prepared to account for medium dilution by the inoculum. Dispense
in tubes containing inverted fermentation vial (Durham’s tube) as desired. Sterilize by autoclaving at 15 lbs pressure (121°C)
for 15 minutes.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on
the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump
formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation.
Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly
after use. Product performance is best if used within stated expiry period.
