Lactobacillus MRS Broth, Granulated (MRS Broth, Granulated) (GM369)
Intended Use:
Recommended for cultivation of all Lactobacilli from clinical and non clinical samples.
Composition
Ingredients g/ L
Proteose peptone 10.000
HM Peptone B# 10.000
Yeast extract 5.000
Dextrose(Glucose) 20.000
Polysorbate 80 (Tween 80) 1.000
Ammonium citrate 2.000
Sodium acetate 5.000
Magnesium sulphate 0.100
Manganese sulphate 0.050
Dipotassium hydrogen phosphate 2.000
Final pH ( at 25°C) 6.5±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef Extract
Directions
Suspend 55.15 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Distribute
in tubes, bottles or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Storage and Shelf Life
Store dehydrated and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be
properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the
product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes
of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within
stated expiry period.
