Hektoen Enteric Agar (M467)
Intended Use:
Recommended for the isolation of Shigella and Salmonella species from enteric pathological specimens.
Composition
Ingredients g/ L
Proteose peptone 12.000
Yeast extract 3.000
Lactose 12.000
Sucrose 12.000
Salicin 2.000
Bile salts mixture 9.000
Sodium chloride 5.000
Sodium thiosulphate 5.000
Ferric ammonium citrate 1.500
Acid fuchsin 0.100
Bromothymol blue 0.065
Agar 15.000
Final pH ( at 25°C) 7.5±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 76.67 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on
the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump
formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation.
Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly
after use. Product performance is best if used within stated expiry period.
