GC Agar Base (M434)
Intended Use:
For selective isolation and cultivation of Gonococci.
Composition
Ingredients g/ L
Peptone, special 15.000
Corn starch 1.000
Dipotassium hydrogen phosphate 4.000
Potassium dihydrogen phosphate 1.000
Sodium chloride 5.000
Agar 10.000
Final pH ( at 25°C) 7.2±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 36.0 grams in 500 ml purified / distilled water, to make a double strength base. Heat to boiling to dissolve the
medium completely. Separately suspend 10 grams of FO Growth Supplement (FD022) in 500 ml purified / distilled
water (2% solution) to make a uniform solution . Separately sterilize both the solutions by autoclaving at 15 lbs pressure
(121°C) for 15 minutes. Cool to 45-50°C. Mix both the solutions uniformly and aseptically add the rehydrated contents
of two vials of GC Selective Supplement (FD021). Mix well and pour into sterile Petri plates.
To increase the selectivity of medium antibiotic supplements such as V.C.N. Supplement (FD023- two vials per litre)
or V.C.N.T. Supplement (FD024 – two vials per litre) or Linco T Supplement (FD026 – two vials per litre) or Vanclo
T Supplement (FD028 – two vials per litre) may be added. To enhance the nutritional properties of medium,
Vitamino Growth Supplement (FD025- two vials per litre) or Yeast Autolysate Supplement (FD027 – two vials per
litre) may be added.
For Chocolate Blood Agar, prepare single-strength medium using 3.6 grams in 100 ml of distilled water. Sterilize
by autoclaving at 15 lbs pressure (121°C) for 15 minutes and add 5% v/v defibrinated blood. Mix well and heat at 80°C
for 10 minutes.
Storage and Shelf Life
Store below 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On
opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the
hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area
protected from extremes of temperature and sources of ignition Seal the container tightly after use.
Product performance is best if used within stated expiry period.
