EMB Broth (M503)
Intended Use:
Recommended for the differentiation of Gram-negative enteric bacteria from clinical & non-clinical specimens.
Composition
Ingredients g/ L
Peptone 10.000
Lactose 5.000
Sucrose 5.000
Dipotassium hydrogen phosphate 2.000
Eosin – Y 0.400
Methylene blue 0.065
Final pH ( at 25°C) 7.2±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 22.46 grams in 1000 ml purified/distilled water. Mix until suspension is uniform. Heat if necessary to dissolve the
medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
AVOID OVERHEATING. Cool to 45-50°C and shake the medium in order to oxidize the methylene blue (i.e. to restore
its blue colour) and to suspend the flocculent precipitate.
Precaution:Store the medium away from light to avoid photooxidation
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on
the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump
formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation.
Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after
use. Product performance is best if used within stated expiry period.
