Deoxycholate Citrate Agar (M065)
Intended Use:
Recommended for the isolation of enteric pathogens particularly Salmonella and Shigella species from clinical samples
Composition
Ingredients g/ L
HI solids # 10.000
Proteose peptone 10.000
Lactose 10.000
Sodium deoxycholate 5.000
Neutral red 0.020
Sodium citrate 20.000
Ferric ammonium citrate 2.000
Agar 13.500
Final pH ( at 25°C) 7.5±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Heart Infusion solids
Directions
Suspend 70.52 grams in 1000 ml of purified / distilled water. Heat to boiling to dissolve the medium
completely. DO NOT AUTOCLAVE. Avoid excessive heating as it is detrimental to the medium. Cool to 45-50°C. Mix
well and pour into sterile Petri plates
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on
the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump
formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump
formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the
container tightly after use. Product performance is best if used within stated expiry period.
