Orange Serum Agar , 500G , Himedia™

Orange Serum Agar                           ( M933 )

Intended use 
Recommended for cultivation and enumeration of microorganisms associated with the spoilage of citrus products, cultivation of Lactobacilli, other aciduric organisms and pathogenic fungi from food samples.

Composition**
Ingredients                                                     Gms / Litre
Tryptone                                                             10.000
Yeast extract                                                        3.000
Dextrose (Glucose)                                             4.000
Dipotassium hydrogen phosphate                      2.500
Orange serum (Solids from 200 ml)                   9.000
Agar                                                                  17.000
Final pH ( at 25°C)                                            5.5±0.2
**Formula adjusted, standardized to suit performance parameters

Directions 
Suspend 45.5 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING. Cool to 45-50°C. Mix well and pour into sterile Petri plates.

Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.7% agar gel.
Colour and Clarity of prepared medium
Medium to dark amber coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.55% w/v aqueous solution at 25°C. pH : 5.5±0.2
pH
5.30-5.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 40-48 hours.(Fungal species are incubated at 25-30°C )

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.