Nitrate Broth , 500G , Himedia™

Nitrate Broth                                              ( M439 )

Intended use 
Recommended for detection of nitrate reduction by bacteria. The composition and performance criteria are in accordance with, ISO 7932:1993.

Composition**
Ingredients                                                                  g / L
Peptone                                                                       5.000
HM peptone B #                                                          3.000
Potassium nitrate                                                        1.000
Final pH ( at 25°C)                                                     7.0±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract

Directions 
Suspend 9.00 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes, flasks or as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light amber coloured clear solution in tubes
Reaction
Reaction of 0.9% w/v aqueous solution at 25°C. pH : 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Nitrate reduction observed on addition of 0.5ml of sulphanilic acid (R015) and 0.5ml of α-naphthylamine Solution (R009).

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label.