Motility Test Medium , 500G , Himedia™

Motility Test Medium                                ( M260 )

Intended use 
Recommended for detection of bacterial motility.

Composition**
Ingredients                                                             Gms / Litre
Tryptose                                                                     10.000
Sodium chloride                                                           5.000
Agar                                                                             5.000
Final pH ( at 25°C)                                                     7.2±0.2
**Formula adjusted, standardized to suit performance parameters

Directions 
Suspend 20 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow tubed medium to cool to 45-50°C in an upright position.

Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Semisolid, comparable with 0.5% Agar gel.
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent gel forms in tubes as butts
Reaction
Reaction of 2.0% w/v aqueous solution at 25°C. pH : 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35 – 37°C for 18 – 48 hours.

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label.