Motility-Indole-Lysine Medium (MIL Medium) , 500G , Himedia™
Used for identification of members of Enterobacteriaceae on the basis of motility, lysine decarboxylase, lysine deaminase and indole production.
Motility-Indole-Lysine Medium (MIL Medium) ( M847 )
Intended use
Recommended for identification of members of Enterobacteriaceae on the basis of motility, lysine decarboxylase, lysine deaminase and indole production.
*Composition**
Ingredients Gms / Litre
Peptone 10.000
Tryptone 10.000
Yeast extract 3.000
L-Lysine hydrochloride 10.000
Dextrose (Glucose) 1.000
Ferric ammonium citrate 0.500
Bromocresol purple 0.020
Agar 2.000
Final pH ( at 25°C) 6.6±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 36.52 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Dispense into tubes in 5 ml amounts. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubes to 45-50°C in an upright position.
Quality Control
Appearance
Cream to greenish yellow homogeneous free flowing powder
Gelling
Semisolid, comparable with 0.2% Agar gel
Colour and Clarity of prepared medium
Reddish purple coloured clear to slightly opalescent gel forms in tubes as butts
Reaction
Reaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2
pH
6.40-6.80
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20 – 30°C. Use before expiry date on the label.
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