McFarland standard set (Each set contains 1 tube of 0.5, 1, 2, 3, 4 McFarland standard) , Himedia™

McFarland Standard set                              ( R092 )

McFarland standards are used to perform spectrophotometric comparisions of bacterial densities in water, saline or liquid growth medium. It provides laboratory guidance for the standardization of numbers of bacteria for susceptibility testing or other procedure requiring a standardization of the inoculum like growth promotion test (GPT).

Set Contains: 

R092A (Standard 0.5)- 1 tube
R092B (Standard 1)- 1 tube
R092C (Standard 2)- 1 tube
R092D (Standard 3)- 1 tube
R092E (Standard 4)- 1 tube

Directions 

Prepare the inoculum of culture required for testing by using sterile saline. Match the density of the resultant suspension with the density of the desired standard. The standards must be thoroughly mixed on a vortex mixture at the time of use to obtain a uniform suspension. Adjust the density of cell suspension by adding saline if it is more turbid as compared to the desired standard or by adding culture if it is dilute. Check the density of the turbidity by determining the absorbance of 0.5 McFarland standard using a spectrophotometer with a 1 cm light path. The absorbance at 625 nm should be 0.08 to 0.10. The standards should be checked regularly to ensure the density accuracy.

Technical Data Sheet

Technical Data R092