Malt Extract Powder, Refined ( RM004B )
Intended use
Malt Extract Powder, Refined is prepared from aqueous extract of sprouted malt grains and dried at low temperature to preserve nutrients present in the form of carbohydrates and nitrogenous substances. It is highly purified made in solubilized form. Recommended in culture media for cultivation of Fungi.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection.
Follow good microbiological lab practices while handling specimens and culture. Safety guidelines may be referred in individual safety data sheets
Limitations
1. It is biological origin product since variation in colour of powder and clarity may observed.
2. Each lot of the product has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user’s requirement.
3. Individual organisms differ in their growth requirement and may show variable growth patterns on the medium prepared by the product.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature
Quality Control
Appearance
Cream to brownish yellow homogenous free flowing powder
Solubility
Freely soluble in distilled/purified water,insoluble in alcohol.
Clarity
1% w/v aqueous solution remains clear without haziness after autoclaving at 15 lbs pressure (121ºC) for 15 minutes.
pH
pH of 2% w/v aqueous solution at 25ºC 4.5-6.5
Microbial Load
Bacterial Count : <= 2000 CFU/gram by plate method, when incubated at 30-35ºC for not less than 3 days
Yeast & mould Count : <= 100 CFU/gram by plate method, when incubated at 20-25ºC for not less than 5 days.
Test for pathogens
1. Escherichia coli- Absent/gram of sample
2. Salmonella species- Absent/10 gram of sample
3. Pseudomonas aeruginosa- Absent/gram of sample
4. Staphylococcus aureus- Absent/gram of sample
5. Candidaalbicans- Absent/gram of sample
6. Clostridia- Absent/gram of sample
Cultural response
Cultural response observed after an incubation at 25-30ºC for 48-72 hours by preparing Malt Extract Agar (M137) using Malt Extract Powder, Refined as an ingredient.
Storage and Shelf Life
Store between 10-30°C in tightly closed container and away from bright light. Use before expiry date on label.