MacConkey Broth w/ Neutral Red , 500g , Himedia™

MacConkey Broth w/ Neutral Red                              ( M007 )

Intended use 
Recommended for selective enrichment and enumeration of coliforms.

*Composition**
Ingredients                                                      g / L
Peptone                                                          20.000
Lactose                                                           10.000
Bile salts                                                           5.000
Sodium chloride                                               5.000
Neutral red                                                       0.075
Final pH ( at 25°C)                                         7.4±0.2

**Formula adjusted, standardized to suit performance parameters

Directions 
Suspend 40.07 grams in 1000 ml purified/ distilled water. Heat if necessary to dissolve the medium completely. Distribute into tubes with inverted Durhams tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubes before inoculation.

Quality Control
Appearance
Pale yellow to pink homogeneous free flowing powder
Colour and Clarity of prepared medium
Red coloured clear solution without any precipitate
Reaction
Reaction of 4.01% w/v aqueous solution at 25°C. pH : 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label.