MacConkey Agar w/ 0.15% Bile Salts, CV and NaCl , 500g , Himedia™
For the selective isolation and differentiation of coliform organisms and other enteric pathogens from clinical and non-clinical samples.
MacConkey Agar w/ 0.15% Bile Salts, CV and NaCl ( M081 )
Intended use
For the selective isolation and differentiation of coliform organisms and other enteric pathogens from clinical and non clinical samples.
*Composition**
Ingredients g / L
Gelatin peptone 17.000
Tryptone 1.500
Peptone 1.500
Lactose 10.000
Bile salts 1.500
Sodium chloride 5.000
Neutral red 0.030
Crystal violet 0.001
Agar 15.000
Final pH ( at 25°C) 7.1±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 51.53 grams in 1000 ml purified/distilled water. Heat to boiling with gentle swirling to dissolve the agar completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45-50°C. Mix well and pour into sterile Petri plates. The surface of the medium should be dry when inoculated.
Quality Control
Appearance
Light yellow to pink homogeneous free flowing powder
Gelling
Firm comparable with 1.5% Agar gel.
Colour and Clarity of prepared medium
Red with purplish tinge coloured clear to slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 5.15% w/v aqueous solution at 25°C. pH : 7.1±0.2
pH
6.90-7.30
Cultural Response
Cultural response was observed after an incubation at 30-35°C for 18-72 hours. Recovery rate is considered as 100% for bacteria growth on Soybean Casein Digest Agar.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label.
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