MacConkey Agar Base , 500g , Himedia™
For studying fermentation reactions of coliforms by adding carbohydrates either individually or in combination.
MacConkey Agar Base ( M1024 )
Intended use
Recommended for studying carbohydrate fermentation reactions of coliforms by adding carbohydrates either individually or in combination.
*Composition**
Ingredients g / L
Peptone 17.000
Proteose peptone 3.000
Bile salts 1.500
Sodium chloride 5.000
Neutral red 0.030
Crystal violet 0.001
Agar 13.500
Final pH ( at 25°C) 7.1±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 40.03 grams in 1000 ml purified/distilled water. Add desired amount of carbohydrate either individually or in combination. Heat to boiling with gentle swirling to dissolve the agar completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45-50°C. Mix well and pour into sterile petri plates. The surface of the medium should be dry when inoculated.
Quality Control
Appearance
Light yellow to pink homogeneous free flowing powder
Gelling
Firm comparable with 1.35% Agar gel
Colour and Clarity of prepared medium
Red with purplish tinge clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.0% w/v aqueous solution at 25°C. pH : 7.1±0.2
pH
6.90-7.30
Cultural Response
Cultural characteristics observed with added 1% lactose, after an incubation at 35-37°C for 18-24 hours.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. For better performance it is advised to store the plates at 2-8°C. Use before expiry date on the label.
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