Lysine Iron Agar , 500g , Himedia™

Lysine Iron Agar                              ( M377 )

Intended use 
Recommended for the differentiation of enteric organisms especially Salmonella Arizonae based on their ability to decarboxylate or deaminate lysine and to form hydrogen sulphide (H2S).The composition of this medium is in accordance with FDA BAM and APHA.

*Composition**
Ingredients                                                  Gms / Litre
Peptone                                                              5.000
Yeast extract                                                       3.000
Dextrose (Glucose)                                             1.000
L-Lysine                                                             10.000
Ferric ammonium citrate                                     0.500
Sodium thiosulphate                                           0.040
Bromocresol purple                                             0.020
Agar                                                                   15.000
Final pH ( at 25°C)                                              6.7±0.2

**Formula adjusted, standardized to suit performance parameters

Directions 
Suspend 34.56 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Dispense into tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubes in slanted position to form slants with deep butts.

Quality Control
Appearance
Light yellow to greyish yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Purple coloured, clear to slightly opalescent gel forms in tubes as slants
Reaction
Reaction of 3.45% w/v aqueous solution at 25°C. pH : 6.7±0.2
pH
6.50-6.90
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours .

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on
the label.