Luria Bertani Broth, Miller (Miller Luria Bertani Broth) , 500g , Himedia™

Luria Bertani Broth, Miller (Miller Luria Bertani Broth)        ( M1245 )

Intended use
Recommended for the cultivation and maintenance of recombinant strains of Escherichia coli for genetic and molecular biology studies; may be used for routine cultivation and estimation of not particularly fastidious microorganisms.

Composition**
Ingredients                                                  Gms / Litre
Tryptone                                                           10.000
Yeast extract                                                      5.000
Sodium chloride                                               10.000
Final pH (at 25°C)                                            7.0±0.2
** Formula adjusted, standardized to suit performance parameters

Directions 
Suspend 25 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Yellow to amber coloured clear solution in tubes
Reaction
Reaction of 2.5% w/v aqueous solution at 25°C. pH : 7.5±0.2
pH
7.30-7.70
Cultural Response
Cultural characteristics observed after an incubation at 35 – 37°C for 18 – 24 hours.

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C.
Use before expiry date on the label.