Lauryl Sulphate Broth (Lauryl Tryptose Broth) , 500g , Himedia™

Lauryl Sulphate Broth (Lauryl Tryptose Broth)    ( M080 )

Intended use
Recommended for detection and enumeration of coliform bacteria in water, waste water, dairy products ,other food samples.

Composition**
Ingredients                                                  Gms / Litre
Tryptose                                                            20.000
Lactose                                                               5.000
Sodium chloride                                                  5.000
Dipotassium hydrogen phosphate                      2.750
Potassium dihydrogen phosphate                      2.750
Sodium lauryl sulphate (SLS)                             0.100
Final pH ( at 25°C)                                             6.8±0.2

**Formula adjusted, standardized to suit performance parameters

Directions
Suspend 35.60 gram in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Distribute into tubes containing inverted Durhams tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For inoculum of 1 ml or less, use single strength medium. For inocula of 10 ml or more, double strength or proportionate medium should be prepared.

Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light yellow coloured, clear solution without any precipitate
Reaction
Reaction of 3.56% w/v aqueous solution at 25°C. pH : 6.8±0.2
pH
6.60-7.00
Cultural Response
Productivity : Cultural characteristics observed after an incubation at 30 ± 1°C for 24 ± 2 to 48 ± 2 hours.
Selectivity : Cultural characteristics observed after an incubation at 30 ± 1°C for 24 ± 2 to 48 ± 2 hours.

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label.