Lactobacillus MRS Agar, Granulated (MRS Agar) , 500g , Himedia™
MRS Agar is recommended for isolation and cultivation of Lactobacilli. Proteose peptone, HM Ppetone B, yeast extract , dextrose provides carbon and nitrogen sources for lactobacilli growth. Sodium acetate and ammonium citrate inhibit Streptococci, moulds and many other microorganisms. Magnesium sulphate and manganese sulphate provide essential ions for multiplication of lactobacilli. Phosphates provide good buffering action in the media. Polysorbate 80 supplies fatty acids required for the metabolism of Lactobacilli.
Lactobacillus MRS Agar ( GM641 )
Intended use
Recommended for isolation and cultivation of all Lactobacillus species
Composition**
Ingredients Gms / Litre
Proteose peptone 10.000
HM Peptone B # 10.000
Yeast extract 5.000
Dextrose (Glucose) 20.000
Tween 80 (Polysorbate 80) 1.000
Ammonium citrate 2.000
Sodium acetate 5.000
Magnesium sulphate 0.100
Manganese sulphate 0.050
Dipotassium hydrogen phosphate 2.000
Agar 12.000
Final pH ( at 25°C) 6.5±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Suspend 67.15 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Quality Control
Appearance
Cream to light yellow coloured granular medium.
Gelling
Firm, comparable with 1.2% Agar gel.
Colour and Clarity of prepared medium
Medium to dark amber coloured slightly opalescent gel forms in Petri plates
Reaction
Reaction of 6.71% w/v aqueous solution at 25°C. pH :6.5±0.2
pH
6.30-6.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours or longer.(with 5% CO2)
Storage and Shelf Life
Store dehydrated powder and the prepared medium at 2-8°C. Use before expiry date on the label.
Related products
