Kovac’s Indole Reagent (R008)
Intended Use:
Kovacs’ Indole Reagent is used for detection of presence of indole produced by microorganisms due to tryptophan deamination.
Composition
Ingredients
p-dimethyl amino benzaldehyde 5.0 gm
Amyl alcohol 75.0 ml
Hydrochloric Acid 25.0 ml
**Formula adjusted, standardized to suit performance parameters
Directions
1. Take 5 ml of a 24 – 48 hours old culture of the organism under investigation.
2. Add 0.2 – 0.3 ml of Kovac’s reagent.
3. Observed for a red coloured ring which indicates positive indole test.
Storage and Shelf Life
Store between 10-30°C in tightly closed container and away from bright light. Use before expiry date on label. On
opening, product should be properly stored in dry ventilated area protected from extremes of temperature and
sources of ignition. Seal the container tightly after use