Egg Yolk Emulsion , 100MLX5VL , Himedia™

Egg Yolk Emulsion (100 ml per vial)                       (FD045)

Sterile stabilized emulsion of egg yolk recommended for use in various culture media.

Composition

Ingredients                       Concentration (100 ml per vial)
Egg yolk                                                 30ml
Sterile saline                                          70ml

Directions
Warm up the refrigerated egg yolk emulsion to room temperature. Shake well to attain uniform emulsion. (Since on
refrigeration emulsion has a tendency to form layers or small lumps). Aseptically add 50 ml emulsion in 950 ml of sterile,
molten, cooled (45-50°C) Baird Parker Agar Base M043/ Baird Parker Agar Base M043S/ Baird Parker HiVeg™ Agar
Base MV043/ Baird Parker HiCynth™ Agar MCD043/ Baird Parker Agar (Agar Medium O) ME043/ Baird Parker Agar
(Agar Medium O) M043B/ Baird Parker Agar Base, Granulated GM043I/ Baird Parker Agar Base, Granulated GM043/
Baird Parker Agar Medium (In accordance with IP 1996) MM043/ Baird Parker Agar Medium MU043/ Baird Parker Agar
Base M043I / Mannitol Salt Agar Base M118/Mannitol Salt Agar Base, Granulated GM118/Mannitol Salt HiCynth™ Agar
Base MCD118 / Mannitol Salt HiVeg™ Agar Base MV118/ Baird Parker Agar Base w/Sulpha M1140.
Aseptically add in 475 ml of sterile, molten, cooled (45-50°C) Bacillus Cereus Agar Base M833/ Bacillus Cereus HiVeg™
Agar Base MV833/ Bacillus Cereus HiCynth™ Agar Base MCD833
OR
Aseptically add 100 ml emulsion in 900 ml of sterile, molten, cooled (45-50°C) McClung Toabe Agar Base M387/ McClung
Toabe HiVeg™ Agar Base MV387/K.R.A.N.E.P. Agar Base M583/K.R.A.N.E.P. HiVeg™ Agar Base MV583 / MYP
Agar Base (Phenol Red Egg Yolk Polymyxin Agar Base) M636/ M636S/ MYP HiVeg™ Agar Base (Phenol Red Egg
Yolk Polymyxin HiVeg™ Agar Base MV636/ MYP Agar Base, Granulated (Phenol Red Egg Yolk Polymyxin Agar
Base, Granulated) GM636 / MYP HiCynth™Agar Base (Phenol Red Egg Yolk Polymyxin HiCynth™ Agar Base) MCD636/
KG Agar Base M658/KG HiVeg™ Agar Base MV658/ L.D. Egg Yolk Agar Base M744/ Egg Yolk Agar Base M808 / Egg
Yolk Agar Base, HiVeg™ MV808/ Egg Yolk Agar Base, Modified M1043 / Modified MYP Agar Base M1139/ Bacillus cereus
Selective Agar Base (MYP) ISO 7932 M1139I /Modified MYP HiVeg™ Agar Base MV1139. Aseptically add in 890 ml of
sterile, molten, cooled (45-50°C) TPEY Agar Base M402/ TPEY HiVeg™ Agar Base MV402.
Aseptically add 450 ml of sterile, molten, cooled (45-50°C) in C. botulinum Isolation Agar Base M911/ C. botulinum
Isolation HiVeg™ Agar Base MV911
OR
Aseptically add 25 ml emulsion in 475 ml of sterile, molten, cooled (45-50°C) Perfringens Agar Base T.S.C./S.F.P.AgarBase)
M837/ Perfringens Agar Base, Granulated (Tryptose Sulphite Cycloserine Agar Base, Granulated) (T.S.C./S.F.P. Agar Base,
Granulated) GM837/ Perfringens HiCynth™ Agar Base (T.S.C/S.F.P HiCynth™ Agar Base) MCD837/ Perfringens HiVeg™
Agar Base (T.S.C. / S.F.P. HiVeg™ Agar Base) MV837/ S.F.P. Agar Base M1005/ S.F.P. HiVegTM Agar Base MV1005.
OR
Aseptically add 80 ml emulsion in 920 ml of sterile, molten, cooled (45-50°C) Anaerobic Egg Agar Base M902 / Anaerobic
Egg HiVeg™ Agar Base MV902.
OR
Aseptically add 20 ml emulsion in 90 ml of sterile, molten, cooled (45-50°C) Polymyxin Pyruvate Egg Yolk Mannitol
Bromothymol Blue Agar Base (PEMBA) M1484.
OR
Aseptically add 15 ml emulsion in 420 ml of sterile, molten, cooled (45-50°C) Willis and Hobb’s Medium Base M1375.
OR
Aseptically add 7ml of Emulsion in 93ml of sterile, molten, cooled (45-50°C) Lipovitellin Salt Mannitol Agar Base M627.
OR
Aseptically add 2 vials of CC Difficile Supplement (FD010), 40 ml of Egg Yolk Emulsion (FD045) together with 10 ml
lysed horse blood in 1000 ml of sterile, molten, cooled (45-50°C) Clostridium Brazier Agar Base M1803
OR
Aseptically add 50ml of concetrated Egg yolk emulsion (FD045) and rehydrated contents of 1 vial of LM Selective
Supplement (FD330) in 950 ml of sterile, molten, cooled (45-50°C) L.mono Selective Agar Base (LM Selective Agar Base)
M1994.
Mix well and pour into sterile petri plates

Storage and Shelf Life
Store at 2 – 8°C. Use before expiry date on the label.

Technical Data Sheet (TD)

Safety Data Sheet (SDS)