EE Broth, Mossel (M287)
Intended Use:
Recommended for the selective enrichment of Enterobacteriaceae in bacteriological examination of foods.
Composition
Ingredients g/ L
Peptone 10.000
Dextrose (Glucose) 5.000
Disodium hydrogen phosphate 6.450
Potassium dihydrogen phosphate 2.000
Bile, purified# 20.000
Brilliant green 0.0135
Final pH ( at 25°C) 7.2±0.2
# Equivalent to Ox bile, purified
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 43.46 grams in 1000 ml purified/distilled water. Dispense in tubes or flasks as desired. Stopper with cotton plugs or
loose fitting caps. Heat in free flowing steam or boiling water for 30 minutes. Avoid overheating of the medium. DO NOT
AUTOCLAVE.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15 – 25°C. Use before expiry date on the
label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation
due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry
ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product
performance is best if used within stated expiry period.