Buffered Peptone Water (ISO) (M1494I)
Intended Use:
Recommended as a pre-enrichment medium of Enterobacteriaceae organisms such as Salmonella and Cronobacterium
species from food and animal feeding stuffs, water, milk, milk products and other products. The composition and
performance criteria of this medium are as per the specifications laid down in ISO 6579-1:2017, ISO 6887-1:2017, ISO
21528-1:2017, ISO 22964:2017, ISO19250:2010, ISO 11133:2014 & A1:2018. Recommended for the propagation of fastidious pathogenic cocci and other organisms associated with blood culture work and allied pathological investigations.
Composition
Ingredients g/ L
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Enzymatic digest of casein
Directions
Suspend 20.07 grams(equivalent weight of dehydrated medium) in 1000 ml purified/ distilled water. Heat if necessary to
dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the
label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation
due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry
ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product
performance is best if used within stated expiry period.