Buffered Peptone Water , 500g , Himedia™

Buffered Peptone Water                      (M614)

Intended Use: 
Recommended as a pre-enrichment medium used for increasing the recovery of injured Salmonella species from food prior to selective enrichment and isolation and also from samples.

Composition

Ingredients                                            g/ L
Proteose peptone                                10.000
Sodium chloride                                    5.000
Disodium hydrogen phosphate             3.500
Potassium hydrogen phosphate           1.500
Final pH ( at 25°C)                              7.2±0.2
**Formula adjusted, standardized to suit performance parameters

Directions
Suspend 20.0 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense in 50
ml amounts into tubes or flasks or as desired. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. If desired
aseptically add rehydrated contents of one vial of CCV Supplement (FD247) to 1000 ml of medium for enrichment of
Escherichia coli O157:H7.

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on
the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent
lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump
formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the
container tightly after use. Product performance is best if used within stated expiry period.

Technical Data Sheet (TD)

Safety Data Sheet (SDS)