Brilliant Green Bile Broth, 2%, 500g , Himedia™

Brilliant Green Bile Broth 2%                           (M121)

Intended Use: 
Recommended for detection and confirmation of coliform bacteria in water, waste water, food, milk and dairy products.

Composition

Ingredients                             g / L
Peptone                               10.000
Lactose                                10.000
Bile#                                     20.000
Brilliant green                       0.0133
Final pH ( at 25°C)              7.2±0.2
**Formula adjusted, standardized to suit performance parameters
# – Equivalent to Oxgall

Directions
Suspend 40.01 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Distribute in
fermentation tubes containing inverted Durhams tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
For preparation of double strength it is recommended to heat the dissolved broth (80.02 grams per litre) at 100°C for 30
minutes. Do not autoclave double strength broth.

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label.

Technical Data Sheet (TD)

Safety Data Sheet (SDS)