Brain Heart Infusion Broth (BHI Broth) , 500g , Himedia™

BHI Broth (Brain Heart Infusion Broth)                      (M210)

Intended Use: 
Recommended for the propagation of fastidious pathogenic cocci and other organisms associated with blood culture work and allied pathological investigations.

Composition

Ingredients                                         g/ L
HM infusion powder #                       12.500
BHI powder                                         5.000
Proteose peptone                             10.000
Dextrose (Glucose)                             2.000
Sodium chloride                                  5.000
Disodium hydrogen phosphate          2.500
Final pH ( at 25°C)                            7.4±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Calf brain infusion from

Directions
Suspend 37.0 grams in 1000 ml purified/distilled water. Dispense into bottles or tubes and sterilize by autoclaving
at 15 lbs pressure (121°C) for 15 minutes. For best results, the medium should be used on the day it is prepared,
otherwise, it should be boiled or steamed for a few minutes and then cooled before use.

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date
on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent
lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump
formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container
tightly after use. Product performance is best if used within stated expiry period.

Technical Data Sheet (TD)

Safety Data Sheet (SDS)