Blood Agar Base No. 2 , 500g , Himedia™

Blood Agar Base No. 2

Intended Use: 
After addition of blood, medium permits maximum recovery of Streptococci, Pneumococci and other fastidious pathogenic microorganisms without interfering with their haemolytic reactions.

Composition

(BS) Ingredients                           Gms / Litre
Proteose peptone                               15.000
HL extract #                                          2.500
Yeast extract                                        5.000
Sodium chloride                                   5.000
Agar                                                   15.000
Final pH ( at 25°C)                            7.4±0.2
**Formula adjusted, standardized to suit performance parameters
# -Equivalent to Liver extract

Directions
Suspend 21.25 grams in 500 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45 – 50°C and aseptically add 7% v/v sterile defibrinated blood.
For Brucella species: Add rehydrated contents of 1 vial of NPBCVN Selective Supplement (FD005) to 500 ml sterile molten base.
For Campylobacter species : Add rehydrated contents of 1 vial of Blaser-Wang Selective Supplement (FD006) or Butzler Selective Supplement (FD007) or Skirrow Selective Supplement (FD008) or Minerals Growth Supplement (FD009) to 500 ml sterile molten base.
For Streptococcus species: Add rehydrated contents of 1 vial of NNP Selective Supplement (FD031) to 500 ml sterile molten base. Mix well and pour into sterile Petri plates.

Storage and Shelf Life
Store between 10- 30°C in a tightly closed container and the prepared medium at 2 – 8°C. Use before expiry date on the label.

Technical Data Sheet (TD)

Safety Data Sheet (SDS)

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