Blood Agar Base (Infusion Agar) (M073)
Intended Use:
Recommended for isolation and cultivation of many fastidious pathogenic micro-organisms like Neisseria, Streptococci after addition of blood from clinical and non-clinical specimens.
Composition
(BS) Ingredients Gms / Litre
HM peptone B# 10.000
Tryptose 10.000
Sodium chloride 5.000
Agar 15.000
Final pH ( at 25°C) 7.3±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef Heart peptone
Directions
Suspend 40.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 5% v/v sterile defibrinated blood. Mix well and pour into sterile Petri plates.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2 – 8°C.