Bismuth Sulphite Agar (M027)
Intended Use:
Recommended for selective isolation and enumeration of Salmonella species from food samples. The composition and performance criteria of this medium are as per specifications laid down in ISO 6579-1:2017.
Composition
(BS) Ingredients Gms / Litre
Peptone # 10.000
HM extract ## 5.000
Dextrose (Glucose) 5.000
Disodium hydrogen phosphate, anhydrous 4.000
Ferrous sulphate, anhydrous 0.300
Bismuth sulphite indicator 8.000
Brilliant green 0.025
Agar 20.000
Final pH ( at 25°C) 7.7±0.2
**Formula adjusted, standardized to suit performance parameters
# – Equivalent to Enzymatic digest of animal tissues ## – Equivalent to Meat extract
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C.