Columbia Blood Agar Base , 500g , Himedia™

Columbia Blood Agar Base                       (M144)

Intended Use: 
For preparation of blood agar, chocolate agar and for preparation of various selective and identification media and isolation of organisms from clinical and non clinical samples.

Composition

Ingredients                                            g/ L
Peptone, special                                  23.000
Corn starch                                            1.000
Sodium chloride                                     5.000
Agar                                                     15.000
Final pH ( at 25°C)                              7.3±0.2
**Formula adjusted, standardized to suit performance parameters

Directions
Suspend 44.0 grams of in 1000 ml purified/ distilled water. Heat to boiling to dissolve the medium completely.
Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C before adding heat sensitive compounds.
For Blood Agar: Add 5% v/v sterile defibrinated sheep blood to sterile cool base.
For Chocolate Agar: Add 10% v/v sterile defibrinated sheep blood to sterile cool base. Heat to 80°C for 10 minutes with
constant agitation.
The medium can be made selective by adding different antimicrobials to sterile base.
For Brucella species: Add rehydrated contents of 1 vial of NPBCVN Selective Supplement (FD005) to 500 ml sterile
molten base.
For Campylobacter species: Add rehydrated contents of 1 vial of Blaser-Wang Selective Supplement (FD006) or
Butzler Selective Supplement (FD007) or Skirrow Selective Supplement (FD008) or VTCA Selective Supplement
(FD090) or Butzler VI Selective Supplement (FD106) to 500 ml sterile molten base along with rehydrated contents
of 1 vial of Minerals Growth Supplement (FD009) and 5-7% v/v horse or sheep blood.
For Gardnerella species: Add rehydrated contents of 1 vial of GNA Selective Supplement (FD056) to 500 ml sterile molten
base.
For Cocci: Add rehydrated contents of 1 vial of NC Selective Supplement (FD030) or NNP Selective Supplement
(FD031) or CO Selective Supplement (FD119) to 500 ml sterile molten base.

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to
the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated
area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product
performance is best if used within stated expiry period.

Technical Data Sheet (TD)

Safety Data Sheet (SDS)